研究業績

1999

  • No braiding of Holliday junctions in positively supercoiled DNA molecules.
    Sun W, Mao C, Iwasaki H, Kemper B, Seeman NC.
    J Mol Biol. 1999 Dec 3;294(3):683-99.
    PMID: 10610789

  • Large-scale analysis of the yeast genome by transposon tagging and gene disruption.
    Ross-Macdonald P, Coelho PS, Roemer T, Agarwal S, Kumar A, Jansen R, Cheung KH, Sheehan A, Symoniatis D, Umansky L, Heidtman M, Nelson FK, Iwasaki H, Hager K, Gerstein M, Miller P, Roeder GS, Snyder M.
    Nature. 1999 Nov 25;402(6760):413-8.
    PMID: 10586881

  • Role of walker motif A of RuvB protein in promoting branch migration of holliday junctions. Walker motif a mutations affect Atp binding, Atp hydrolyzing, and DNA binding activities of Ruvb.
    Hishida T, Iwasaki H, Yagi T, Shinagawa H.
    J Biol Chem. 1999 Sep 3;274(36):25335-42.
    PMID: 10464259

  • Novel properties of the Thermus thermophilus RuvB protein, which promotes branch migration of Holliday junctions.
    Yamada K, Fukuoh A, Iwasaki H, Shinagawa H.
    Mol Gen Genet. 1999 Jul;261(6):1001-11.
    PMID: 10485292

1998

  • Mutational analysis on structure-function relationship of a holliday junction specific endonuclease RuvC.
    Ichiyanagi K, Iwasaki H, Hishida T, Shinagawa H.
    Genes Cells. 1998 Sep;3(9):575-86.
    PMID: 9813108

  • Abortive recombination in Escherichia coli ruv mutants blocks chromosome partitioning.
    Ishioka K, Fukuoh A, Iwasaki H, Nakata A, Shinagawa H.
    Genes Cells. 1998 Apr;3(4):209-20.
    PMID: 9663656

  • Functional analyses of the domain structure in the Holliday junction binding protein RuvA.
    Nishino T, Ariyoshi M, Iwasaki H, Shinagawa H, Morikawa K.
    Structure. 1998 Jan 15;6(1):11-21.
    PMID: 9493263

1997

  • Reconstitution, morphology and crystallization of a fatty acid beta-oxidation multienzyme complex from Pseudomonas fragi.
    Ishikawa M, Mikami Y, Usukura J, Iwasaki H, Shinagawa H, Morikawa K.
    Biochem J. 1997 Dec 15;328 ( Pt 3):815-20.
    PMID: 9396725

  • ATP-dependent resolution of R-loops at the ColE1 replication origin by Escherichia coli RecG protein, a Holliday junction-specific helicase.
    Fukuoh A, Iwasaki H, Ishioka K, Shinagawa H.
    EMBO J. 1997 Jan 2;16(1):203-9.
    PMID: 9009281

  • Roles of the recG gene product of Escherichia coli in recombination repair: effects of the delta recG mutation on cell division and chromosome partition.
    Ishioka K, Iwasaki H, Shinagawa H.
    Genes Genet Syst. 1997 Apr;72(2):91-9.
    PMID: 9265736

1996

  • Characterization and comparison of synthetic immobile and mobile Holliday junctions.
    Shida T, Iwasaki H, Shinagawa H, Kyogoku Y.
    J Biochem. 1996 Apr;119(4):653-8.
    PMID: 8743565

  • Analysis of substrate specificity of the RuvC holliday junction resolvase with synthetic Holliday junctions.
    Shida T, Iwasaki H, Saito A, Kyogoku Y, Shinagawa H.
    J Biol Chem. 1996 Oct 18;271(42):26105-9.
    PMID: 8824253

  • Molecular analysis of the Pseudomonas aeruginosa genes, ruvA, ruvB and ruvC, involved in processing of homologous recombination intermediates.
    Hishida T, Iwasaki H, Ishioka K, Shinagawa H.
    Gene. 1996 Dec 5;182(1-2):63-70.
    PMID: 8982068

1995

  • Identification of four acidic amino acids that constitute the catalytic center of the RuvC Holliday junction resolvase.
    Saito A, Iwasaki H, Ariyoshi M, Morikawa K, Shinagawa H.
    Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7470-4.
    PMID: 7638215

1994

  • Atomic structure of the RuvC resolvase: a holliday junction-specific endonuclease from E. coli.
    Ariyoshi M, Vassylyev DG, Iwasaki H, Nakamura H, Shinagawa H, Morikawa K.
    Cell. 1994 Sep 23;78(6):1063-72.
    PMID: 7923356

  • Preliminary crystallographic study of Escherichia coli RuvC protein. An endonuclease specific for Holliday junctions.
    Ariyoshi M, Vassylyev DG, Iwasaki H, Fujishima A, Shinagawa H, Morikawa K.
    J Mol Biol. 1994 Aug 12;241(2):281-2.
    PMID: 8057369

  • Structural requirements of substrate DNA for binding to and cleavage by RuvC, a Holliday junction resolvase.
    Takahagi M, Iwasaki H, Shinagawa H.
    J Biol Chem. 1994 May 27;269(21):15132-9.
    PMID: 8195150

  • Amino acid sequence motifs essential to 3'-->5' exonuclease activity of Escherichia coli DNA polymerase II.
    Ishino Y, Iwasaki H, Kato I, Shinagawa H.
    J Biol Chem. 1994 May 20;269(20):14655-60.
    PMID: 8182073

1993

  • Escherichia coli RuvA and RuvB proteins involved in recombination repair: physical properties and interactions with DNA.
    Shiba T, Iwasaki H, Nakata A, Shinagawa H.
    Mol Gen Genet. 1993 Mar;237(3):395-9.
    PMID: 8483454

1992

  • Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration.
    Iwasaki H, Takahagi M, Nakata A, Shinagawa H.
    Genes Dev. 1992 Nov;6(11):2214-20.
    PMID: 1427081

  • Aphidicolin inhibits DNA polymerizing activity but not nucleolytic activity of Escherichia coli DNA polymerase II.
    Ishino Y, Iwasaki H, Fukui H, Mineno J, Kato I, Shinagawa H.
    Biochimie. 1992 Feb;74(2):131-6.
    PMID: 1581388

1991

  • Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure.
    Iwasaki H, Takahagi M, Shiba T, Nakata A, Shinagawa H.
    EMBO J. 1991 Dec;10(13):4381-9.
    PMID: 1661673

  • SOS-inducible DNA repair proteins, RuvA and RuvB, of Escherichia coli: functional interactions between RuvA and RuvB for ATP hydrolysis and renaturation of the cruciform structure in supercoiled DNA.
    Shiba T, Iwasaki H, Nakata A, Shinagawa H.
    Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8445-9.
    PMID: 1833759

  • Molecular analysis of the Escherichia coli ruvC gene, which encodes a Holliday junction-specific endonuclease.
    Takahagi M, Iwasaki H, Nakata A, Shinagawa H.
    J Bacteriol. 1991 Sep;173(18):5747-53.
    PMID: 1885548

  • Properties of the Escherichia coli RuvA and RuvB proteins involved in DNA repair, recombination and mutagenesis.
    Shinagawa H, Shiba T, Iwasaki H, Makino K, Takahagi M, Nakata A.
    Biochimie. 1991 Apr;73(4):505-7. Review.
    PMID: 1911951

  • SOS-inducible DNA polymerase II of E coli is homologous to replicative DNA polymerase of eukaryotes.
    Shinagawa H, Iwasaki H, Ishino Y, Nakata A.
    Biochimie. 1991 Apr;73(4):433-5. Review.
    PMID: 1911943

  • Escherichia coli DNA polymerase II is homologous to alpha-like DNA polymerases.
    Iwasaki H, Ishino Y, Toh H, Nakata A, Shinagawa H.
    Mol Gen Genet. 1991 Apr;226(1-2):24-33.
    PMID: 2034216

1990

  • The Escherichia coli polB gene, which encodes DNA polymerase II, is regulated by the SOS system.
    Iwasaki H, Nakata A, Walker GC, Shinagawa H.
    J Bacteriol. 1990 Nov;172(11):6268-73.
    PMID: 2228959

  • Proteolytic processing of MucA protein in SOS mutagenesis: both processed and unprocessed MucA may be active in the mutagenesis.
    Shiba T, Iwasaki H, Nakata A, Shinagawa H.
    Mol Gen Genet. 1990 Nov;224(2):169-76.
    PMID: 2277636

  • Overproduction of human immunodeficiency virus type I reverse transcriptase in Escherichia coli and purification of the enzyme.
    Saitoh A, Iwasaki H, Nakata A, Adachi A, Shinagawa H.
    Microbiol Immunol. 1990;34(6):509-21.
    PMID: 1699113

1989

  • Overproduction, purification, and ATPase activity of the Escherichia coli RuvB protein involved in DNA repair.
    Iwasaki H, Shiba T, Makino K, Nakata A, Shinagawa H.
    J Bacteriol. 1989 Oct;171(10):5276-80.
    PMID: 2529252

  • Involvement in DNA repair of the ruvA gene of Escherichia coli.
    Iwasaki H, Shiba T, Nakata A, Shinagawa H.
    Mol Gen Genet. 1989 Oct;219(1-2):328-31.
    PMID: 2693946

1988

  • Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination.
    Shinagawa H, Makino K, Amemura M, Kimura S, Iwasaki H, Nakata A.
    J Bacteriol. 1988 Sep;170(9):4322-9.
    PMID: 2842314

  • RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis.
    Shinagawa H, Iwasaki H, Kato T, Nakata A.
    Proc Natl Acad Sci U S A. 1988 Mar;85(6):1806-10.
    PMID: 3126496

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