Publication・成果

2021

2020

  • Cooperative interactions facilitate stimulation of Rad51 by the Swi5-Sfr1 auxiliary factor complex.
    Argunhan B, Sakakura M, Afshar N, Kurihara M, Ito K, Maki T, Kanamaru S, Murayama Y, Tsubouchi H, Takahashi M, Takahashi H, Iwasaki H.
    Elife. 2020 Mar 24;9. pii: e52566.
    doi: 10.7554/eLife.52566.
    PMID: 32204793. 
  • Rad52-dependent single-strand annealing that leads to gross chromosomal rearrangements at centromeres.
    Onaka AT, Su J, Katahira Y, Tang C, Zafar F, Aoki K, Kagawa W, Niki H, Iwasaki H, Nakagawa T.
    Commun Biol. 2020 Apr 30;3(1):202.
    doi: 10.1038/s42003-020-0934-0.
    PMID: 32355220. 

2019

  • Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51.
    Ito K, Argunhan B, Tsubouchi H, Iwasaki H.
    J Vis Exp. 2019 Feb 13;(144). doi:
    10.3791/59073.
    PMID: 30829327.

2018

  • Mating-type switching by homology-directed recombinational repair: a matter of choice.
    Thon G, Maki T, Haber JE, Iwasaki H.
    Curr Genet. 2018 Oct 31. doi: 10.1007/s00294-018-0900-2.
    PMID: 30382337
  • Swi5-Sfr1 stimulates Rad51 recombinase filament assembly by modulating Rad51 dissociation.
    Lu CH, Yeh HY, Su GC, Ito K, Kurokawa Y, Iwasaki H, Chi P, Li HW.
    Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10059-E10068.
    PMID: 30297419
  • New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
    Maki T, Ogura N, Haber JE, Iwasaki H, Thon G.
    PLoS Genet. 2018 May 31;14(5).
    PMID: 29852001
  • Establishment of DNA-DNA Interactions by the Cohesin Ring.
    Murayama Y, Samora CP, Kurokawa Y, Iwasaki H, Uhlmann F.
    Cell. (2018) 172(3):465-477.
    PMID: 29358048
  • Two three-strand intermediates are processed during Rad51-driven DNA strand exchange
    Ito K, Murayama Y, Takahashi M, Iwasaki H.
    Nat Struct Mol Biol. (2018) 25, 29-36.
    PMID: 29323270

2017

  • Fundamental cell cycle kinases collaborate to ensure timely destruction of the synaptonemal complex during meiosis
    Argunhan B, Leung W K, Afshar N, Terentyev Y, Subramanian V V, Murayama Y, Hochwagen A, Iwasaki H, Tsubouchi T, Tsubouchi H.
    EMBO J. (2017) 36, 2488-2509.
    PMID: 28694245
  • In vitro site-specific recombination mediated by the tyrosine recombinase XerA of Thermoplasma acidophilum.
    Jo M, Murayama Y, Tsutsui Y, Iwasaki H.
    Genes to Cells. (2017) 22(7): 646-661.
    PMID: 28557347
  • The differentiated and conserved roles of Swi5-Sfr1 in homologous recombination.
    Argunhan B, Murayama Y, Iwasaki H.
    FEBS Letters. (2017) 591: 2035-2047.
    PMID: 28423184
  • Holliday junction resolvases mediate chloroplast nucleoid segregation.
    Kobayashi Y, Misumi O, Odahara M, Ishibashi K, Hirono M, Hidaka K, Endo M, Sugiyama H, Iwasaki H, Kuroiwa T, Shikanai T, Nishimura Y.
    Science. (2017) 356(6338), 631-634.
    PMID: 28495749
  • Recruitment and delivery of the fission yeast Rst2 transcription factor
    via a local genome structure counteracts repression by Tup1-family corepressors.
    Asada R, Umeda M, Adachi A, Senmatsu S, Abe T, Iwasaki H, Ohta K, Hoffman CS,
    Hirota K.
    Nucleic Acids Res. 2017 Sep 19;45(16):9361-9371. doi: 10.1093/nar/gkx555.
    PMID: 28934464

2014

  • Multiple regulation of rad51-mediated homologous recombination by fission yeast fbh1.
    Tsutsui Y, Kurokawa Y, Ito K, Siddique MS, Kawano Y, Yamao F, Iwasaki H.
    PLoS Genet. (2014) 10(8):e1004542.
    PMID: 25165823
  • Swi5-Sfr1 protein stimulates Rad51-mediated DNA strand exchange reaction through organization of DNA bases in the presynaptic filament.
    Fornander LH, Renodon-Corniere A, Kuwabara N, Ito K, Tsutsui Y, Shimizu T, Iwasaki H, Norden B, Takahashi M.
    Nucleic Acids Res. (2014) 42: 2358-2365.
    PMID: 24304898

2013

  • Dual regulation of Dmc1-driven DNA strand exchange by Swi5-Sfr1 activation and Rad22 inhibition.
    Murayama Y, Kurokawa Y, Tsutsui Y, Iwasaki H.
    Genes Dev. (2013) 27:2299-2304.
    PMID: 24186976
  • Characterisation of an intrinsically disordered protein complex of Swi5-Sfr1 by ion mobility mass spectrometry and small-angle X-ray scattering.
    Saikusa K, Kuwabara N, Kokabu Y, Inoue Y, Sato M, Iwasaki H, Shimizu T, Ikeguchi M, and Akashi S.
    Analyst. (2013) 138:1441-1449.
    PMID: 23324799

2012

  • Mechanistic insights into the activation of rad51-mediated strand exchange from the structure of a recombination activator, the swi5-sfr1 complex.
    Kuwabara N, Murayama Y, Hashimoto H, Kokabu Y, Ikeguchi M, Sato M, Mayanagi K, Tsutsui Y, Iwasaki H, Shimizu T.
    Structure. 2012 Mar 7;20(3):440-9.
    PMID: 22405003
  • RNA interference regulates the cell cycle checkpoint through the RNA export factor, Ptr1, in fission yeast.
    Iida T, Iida N, Tsutsui Y, Yamao F, Kobayashi T.
    Biochem Biophys Res Commun. (2012) 427(1):143-147.
    PMID: 22989756

2011

  • Fission yeast Swi5-Sfr1 protein complex, an activator of Rad51 recombinase, forms an extremely elongated dogleg-shaped structure.
    Kokabu Y, Murayama Y, Kuwabara N, Oroguchi T, Hashimoto H, Tsutsui Y, Nozaki N, Akashi S, Unzai S, Shimizu T, Iwasaki H, Sato M, Ikeguchi M.
    J Biol Chem. 2011 Dec 16;286(50):43569-76. Epub 2011 Oct 27.
    PMID: 22033972
  • Cell polarity in Saccharomyces cerevisiae depends on proper localization of the Bud9 landmark protein by the EKC/KEOPS complex.
    Kato Y, Kawasaki H, Ohyama Y, Morishita T, Iwasaki H, Kokubo T, Hirano H.
    Genetics. 2011 Aug;188(4):871-82. Epub 2011 May 30.
    PMID: 21625000
  • An in vitro assay for monitoring the formation and branch migration of hollidayjunctions mediated by a eukaryotic recombinase.
    Murayama Y, Iwasaki H.
    Methods Mol Biol. 2011;745:385-405.
    PMID: 21660706
  • Opposing role of condensin hinge against replication protein A in mitosis and interphase through promoting DNA annealing.
    Akai Y, Kurokawa Y, Nakazawa N, Tonami-Murakami, Y, Yoshimura S H, Iwasaki H, Shiroiwa Y, Nakamura T, Shibata E, Yanagida M.
    Open Biol. (2011) 1. e110023 doi: 10.1098/rsob.110023
  • The fission yeast meiosis-specific Dmc1 recombinase mediates formation and branch migration of Holliday junctions by preferentially promoting strand exchange in a direction opposite to that of Rad51.
    Murayama Y, Tsutsui Y, Iwasaki H.
    Genes Dev. 2011 Mar 1;25(5):516-27.
    PMID: 19079240

2010

  • Expression, purification and crystallization of Swi5 and the Swi5-Sfr1 complex from fission yeast.
    Kuwabara N, Hashimoto H, Yamada N, Unzai S, Ikeguchi M, Sato M, Murayama Y, Iwasaki H, Shimizu T.
    Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Sep 1;66(Pt 9):1124-6.
    PMID: 20823543
  • Srs2 plays a critical role in reversible G2 arrest upon chronic and low doses of UV irradiation via two distinct homologous recombination-dependent mechanisms in postreplication repair-deficient cells.
    Hishida T, Hirade Y, Haruta N, Kubota Y, Iwasaki H.
    Mol Cell Biol. 2010 Oct;30(20):4840-50.
    PMID: 20713444
  • DDK phosphorylates checkpoint clamp component Rad9 and promotes its release from damaged chromatin.
    Furuya K, Miyabe I, Tsutsui Y, Paderi F, Kakusho N, Masai H, Niki H, Carr AM.
    Mol Cell. (2010) 40(4):606-618.
    PMID: 21095590

2009

  • Nbs1 flexibly tethers Ctp1 and Mre11-Rad50 to coordinate DNA double-strand break processing and repair.
    Williams RS, Dodson GE, Limbo O, Yamada Y, Williams JS, Guenther G, Classen S, Glover JN, Iwasaki H, Russell P, Tainer JA.
    Cell. 2009 Oct 2;139(1):87-99.
    PMID: 19804755
  • RAD6-RAD18-RAD5-pathway-dependent tolerance to chronic low-dose ultraviolet light.
    Hishida T, Kubota Y, Carr AM, Iwasaki H.
    Nature. 2009 Jan 29;457(7229):612-5.
    PMID: 19079240

2008

  • A DNA polymerase alpha accessory protein, Mcl1, is required for propagation of centromere structures in fission yeast.
    Natsume T, Tsutsui Y, Sutani T, Dunleavy EM, Pidoux AL, Iwasaki H, Shirahige K, Allshire RC, Yamao F.
    PLoS ONE. 2008 May 21;3(5):e2221.
    PMID: 18493607
  • Molecular characterization of the role of the Schizosaccharomyces pombe nip1+/ctp1+ gene in DNA double-strand break repair in association with the Mre11-Rad50-Nbs1 complex.
    Akamatsu Y, Murayama Y, Yamada T, Nakazaki T, Tsutsui Y, Ohta K, Iwasaki H.
    Mol Cell Biol. 2008 Jun;28(11):3639-51.
    PMID: 18378696
  • Mus81 is essential for sister chromatid recombination at broken replication forks.
    Roseaulin L, Yamada Y, Tsutsui Y, Russell P, Iwasaki H, Arcangioli B.
    EMBO J. 2008 May 7;27(9):1378-87.
    PMID: 18388861
  • Reconstitution of DNA strand exchange mediated by Rhp51 recombinase and two mediators.
    Kurokawa Y, Murayama Y, Haruta-Takahashi N, Urabe I, Iwasaki H.
    PLoS Biol. 2008 Apr 15;6(4):e88.
    PMID: 18416603
  • Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases.
    Murayama Y, Kurokawa Y, Mayanagi K, Iwasaki H.
    Nature. 2008 Feb 21;451(7181):1018-21.
    PMID: 18256600

2007

  • Fission yeast Swi5/Sfr1 and Rhp55/Rhp57 differentially regulate Rhp51-dependent recombination outcomes.
    Akamatsu Y, Tsutsui Y, Morishita T, Siddique MS, Kurokawa Y, Ikeguchi M, Yamao F, Arcangioli B, Iwasaki H.
    EMBO J. 2007 Mar 7;26(5):1352-62.
    PMID: 17304215

2006

  • The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro.
    Haruta N, Kurokawa Y, Murayama Y, Akamatsu Y, Unzai S, Tsutsui Y, Iwasaki H.
    Nat Struct Mol Biol. 2006 Sep;13(9):823-30.
    PMID: 16921379
  • Direct observation of DNA rotation during branch migration of Holliday junction DNA by Escherichia coli RuvA-RuvB protein complex.
    Han YW, Tani T, Hayashi M, Hishida T, Iwasaki H, Shinagawa H, Harada Y.
    Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11544-8.
    PMID: 16864792

2005

  • Role of the Schizosaccharomyces pombe F-Box DNA helicase in processing recombination intermediates.
    Morishita T, Furukawa F, Sakaguchi C, Toda T, Carr AM, Iwasaki H, Shinagawa H.
    Mol Cell Biol. 2005 Sep;25(18):8074-83
    PMID: 16135799
  • Structure-function analysis of the three domains of RuvB DNA motor protein.
    Ohnishi T, Hishida T, Harada Y, Iwasaki H, Shinagawa H.
    J Biol Chem. 2005 Aug 26;280(34):30504-10.
    PMID: 15972826
  • Genetic and physical interactions between Schizosaccharomyces pombe Mcl1 and Rad2, Dna2 and DNA polymerase alpha: evidence for a multifunctional role of Mcl1 in DNA replication and repair.
    Tsutsui Y, Morishita T, Natsume T, Yamashita K, Iwasaki H, Yamao F, Shinagawa H.
    Curr Genet. 2005 Jul;48(1):34-43.
    PMID: 15915339
  • Functional overlap between RecA and MgsA (RarA) in the rescue of stalled replication forks in Escherichia coli.
    Shibata T, Hishida T, Kubota Y, Han YW, Iwasaki H, Shinagawa H.
    Genes Cells. 2005 Mar;10(3):181-91.
    PMID: 15743409

2004

  • Rad62 protein functionally and physically associates with the smc5/smc6 protein complex and is required for chromosome integrity and recombination repair in fission yeast.
    Morikawa H, Morishita T, Kawane S, Iwasaki H, Carr AM, Shinagawa H.
    Mol Cell Biol. 2004 Nov;24(21):9401-13.
    PMID: 15485909
  • Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks.
    Hishida T, Han YW, Shibata T, Kubota Y, Ishino Y, Iwasaki H, Shinagawa H.
    Genes Dev. 2004 Aug 1;18(15):1886-97.
    PMID: 15289460
  • Role of the RuvAB protein in avoiding spontaneous formation of deletion mutations in the Escherichia coli K-12 endogenous tonB gene.
    Mashimo K, Nagata Y, Kawata M, Iwasaki H, Yamamoto K.
    Biochem Biophys Res Commun. 2004 Oct 8;323(1):197-203.
    PMID: 15351721
  • Direct evidence that a conserved arginine in RuvB AAA+ ATPase acts as an allosteric effector for the ATPase activity of the adjacent subunit in a hexamer.
    Hishida T, Han YW, Fujimoto S, Iwasaki H, Shinagawa H.
    Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9573-7. Epub 2004 Jun 21.
    PMID: 15210950

2003

  • Two different Swi5-containing protein complexes are involved in mating-type switching and recombination repair in fission yeast.
    Akamatsu Y, Dziadkowiec D, Ikeguchi M, Shinagawa H, Iwasaki H.
    Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15770-5. Epub 2003 Dec 8.
    PMID: 14663140
  • Molecular characterization of the Schizosaccharomyces pombe nbs1+ gene involved in DNA repair and telomere maintenance.
    Ueno M, Nakazaki T, Akamatsu Y, Watanabe K, Tomita K, Lindsay HD, Shinagawa H, Iwasaki H.
    Mol Cell Biol. 2003 Sep;23(18):6553-63.
    PMID: 12944481
  • Uncoupling of the ATPase activity from the branch migration activity of RuvAB protein complexes containing both wild-type and ATPase-defective RuvB proteins.
    Hishida T, Iwasaki H, Han YW, Ohnishi T, Shinagawa H.
    Genes Cells. 2003 Sep;8(9):721-30.
    PMID: 12940820
  • Competition between the Rad50 complex and the Ku heterodimer reveals a role for Exo1 in processing double-strand breaks but not telomeres.
    Tomita K, Matsuura A, Caspari T, Carr AM, Akamatsu Y, Iwasaki H, Mizuno K, Ohta K, Uritani M, Ushimaru T, Yoshinaga K, Ueno M.
    Mol Cell Biol. 2003 Aug;23(15):5186-97
    PMID: 12861005

2002

  • Crystal structure of the RuvA-RuvB complex: a structural basis for the Holliday junction migrating motor machinery.
    Yamada K, Miyata T, Tsuchiya D, Oyama T, Fujiwara Y, Ohnishi T, Iwasaki H, Shinagawa H, Ariyoshi M, Mayanagi K, Morikawa K.
    Mol Cell. 2002 Sep;10(3):671-81.
    PMID: 12408833
  • Parallel symmetric immobile DNA junctions as substrates for E. coli RuvC Holliday junction resolvase.
    Sha R, Liu F, Iwasaki H, Seeman NC.
    Biochemistry. 2002 Sep 10;41(36):10985-93.
    PMID: 12206670
  • 1-Methyl-4-phenylpyridinium ion, a toxin that can cause parkinsonism, alters branched structures of DNA.
    Iwaasa M, Umeda S, Ohsato T, Takamatsu C, Fukuoh A, Iwasaki H, Shinagawa H, Hamasaki N, Kang D.
    J Neurochem. 2002 Jul;82(1):30-7.
    PMID: 12091462
  • The Schizosaccharomyces pombe rad60 gene is essential for repairing double-strand DNA breaks spontaneously occurring during replication and induced by DNA-damaging agents.
    Morishita T, Tsutsui Y, Iwasaki H, Shinagawa H.
    Mol Cell Biol. 2002 May;22(10):3537-48.
    PMID: 11971984
  • Saccharomyces cerevisiae MGS1 is essential in strains deficient in the RAD6-dependent DNA damage tolerance pathway.
    Hishida T, Ohno T, Iwasaki H, Shinagawa H.
    EMBO J. 2002 Apr 15;21(8):2019-29.
    PMID: 11953321
  • p53 blocks RuvAB promoted branch migration and modulates resolution of Holliday junctions by RuvC.
    Prabhu VP, Simons AM, Iwasaki H, Gai D, Simmons DT, Chen J.
    J Mol Biol. 2002 Mar 8;316(5):1023-32
    PMID: 11884140

2001

  • Multiple interactions among the components of the recombinational DNA repair system in Schizosaccharomyces pombe.
    Tsutsui Y, Khasanov FK, Shinagawa H, Iwasaki H, Bashkirov VI.
    Genetics. 2001 Sep;159(1):91-105.
    PMID: 11560889
  • A unique beta-hairpin protruding from AAA+ ATPase domain of RuvB motor protein is involved in the interaction with RuvA DNA recognition protein for branch migration of Holliday junctions.
    Han YW, Iwasaki H, Miyata T, Mayanagi K, Yamada K, Morikawa K, Shinagawa H.
    J Biol Chem. 2001 Sep 14;276(37):35024-8. Epub 2001 Jun 26.
    PMID: 11427534
  • A yeast gene, MGS1, encoding a DNA-dependent AAA(+) ATPase is required to maintain genome stability.
    Hishida T, Iwasaki H, Ohno T, Morishita T, Shinagawa H.
    Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8283-9.
    PMID: 11459965
  • Mutagenic and nonmutagenic bypass of DNA lesions by Drosophila DNA polymerases dpoleta and dpoliota.
    Ishikawa T, Uematsu N, Mizukoshi T, Iwai S, Iwasaki H, Masutani C, Hanaoka F, Ueda R, Ohmori H, Todo T.
    J Biol Chem. 2001 May 4;276(18):15155-63. Epub 2001 Jan 31.
    PMID: 11297519
  • Evidence that phenylalanine 69 in Escherichia coli RuvC resolvase forms a stacking interaction during binding and destabilization of a Holliday junction DNA substrate.
    Yoshikawa M, Iwasaki H, Shinagawa H.
    J Biol Chem. 2001 Mar 30;276(13):10432-6. Epub 2001 Jan 10.
    PMID: 11152689
  • Crystal structure of the Holliday junction migration motor protein RuvB from Thermus thermophilus HB8.
    Crystal structure of the Holliday junction migration motor protein RuvB from Thermus thermophilus HB8.
    Yamada K, Kunishima N, Mayanagi K, Ohnishi T, Nishino T, Iwasaki H, Shinagawa H, Morikawa K.
    Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1442-7.
    PMID: 11171970

2000

  • Identification and characterization of Thermus thermophilus HB8 RuvA protein, the subunit of the RuvAB protein complex that promotes branch migration of Holliday junctions.
    Ohnishi T, Iwasaki H, Ishino Y, Kuramitsu S, Nakata A, Shinagawa H.
    Genes Genet Syst. 2000 Oct;75(5):233-43.
    PMID: 11245216
  • Two basic residues, Lys-107 and Lys-118, of RuvC resolvase are involved in critical contacts with the Holliday junction for its resolution.
    Yoshikawa M, Iwasaki H, Kinoshita K, Shinagawa H.
    Genes Cells. 2000 Oct;5(10):803-13.
    PMID: 11029656
  • Two different oligomeric states of the RuvB branch migration motor protein as revealed by electron microscopy.
    Miyata T, Yamada K, Iwasaki H, Shinagawa H, Morikawa K, Mayanagi K.
    J Struct Biol. 2000 Aug;131(2):83-9.
    PMID: 11042078
  • Cleavage of symmetric immobile DNA junctions by Escherichia coli RuvC.
    Sha R, Iwasaki H, Liu F, Shinagawa H, Seeman NC.
    Biochemistry. 2000 Oct 3;39(39):11982-8.
    PMID: 11009612
  • Crystal structure of the holliday junction DNA in complex with a single RuvA tetramer.
    Ariyoshi M, Nishino T, Iwasaki H, Shinagawa H, Morikawa K.
    Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8257-62.
    PMID: 10890893
  • Modulation of RuvB function by the mobile domain III of the Holliday junction recognition protein RuvA.
    Nishino T, Iwasaki H, Kataoka M, Ariyoshi M, Fujita T, Shinagawa H, Morikawa K.
    J Mol Biol. 2000 May 5;298(3):407-16.
    PMID: 10772859
  • Mutational analysis of the functional motifs of RuvB, an AAA+ class helicase and motor protein for holliday junction branch migration.
    Iwasaki H, Han YW, Okamoto T, Ohnishi T, Yoshikawa M, Yamada K, Toh H, Daiyasu H, Ogura T, Shinagawa H.
    Mol Microbiol. 2000 May;36(3):528-38.
    PMID: 10844644
  • A recombination repair gene of Schizosaccharomyces pombe, rhp57, is a functional homolog of the Saccharomyces cerevisiae RAD57 gene and is phylogenetically related to the human XRCC3 gene.
    Tsutsui Y, Morishita T, Iwasaki H, Toh H, Shinagawa H.
    Genetics. 2000 Apr;154(4):1451-61.
    PMID: 10747044
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